The second trial test was taken by 235 students from the six partner universities. The test items covered the four subscales: (1) basic sciences, (2) clinical sciences of companion animals/equines and (3) of production animals, and (4) veterinary public health and food safety. The test contained 28 anchor items (validated items from trial test 1) and 123 new items. After psychometric validation of the question items, 150 out of 151 trialed items were used for calculating students’ test scores. Students that have participated in both the first and second trial have received additional feedback on their progress between the two trials.